Text Box: Abstract:   Potential treatment strategies of neurodegenerative and other diseases with stem cells derived from nonembryonic tissues are much less subjected to ethical criticism than embryonic stem cell-based approaches. Here we report the isolation of inner ear stem cells, which may be useful in cell replacement therapies for hearing loss, after protracted postmortem intervals. We found that neonatal murine inner ear tissues, including vestibular and cochlear sensory epithelia, display remarkably robust cellular survival, even 10 days postmortem. Similarly, isolation of sphere-forming stem cells was possible up to 10 days postmortem. We detected no difference in the proliferation and differentiation potential between stem cells isolated directly after death and up to 5 days postmortem. At longer postmortem intervals, we observed that the potency of sphere-derived cells to spontaneously differentiate into mature cell types diminishes prior to the cells losing their potential for self-renewal. Three-week-old mice also displayed sphere-forming stem cells in all inner ear tissues investigated up to 5 days postmortem. In summary, our results demonstrate that postmortem murine inner ear tissue is suited for isolation of stem cells.  

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Text Box: May  13 2007

Text Box: Otorhinolaryngology News

Text Box: Inside This Issue · LigPost Mortem Survival & Cochlear ES Cells · IMAge, Systemic Dx & Poor prognosis in RH syndrome? · TinOlfacto-Cochlear ESC transplant? *     Case of the Week

Case Review Clinical Photograph of The Week
 

A 53-year-old male was referred from the Urology clinic, where he was awaiting surgery for prostatic carcinoma, on account of a right sided neck mass. Neck examination showed right sided level 2 N3 finding, and oropharyngeal examination revealed the feature displayed on this picture.  >>>>

 

 

 

What are the differential diagnoses?

 

1.    The 9th International Symposium on Recent Advances in Otitis Media on June 3-7, 2007 in St. Pete Beach, Florida. Website address : www.otitis2007.org ;email address for information and questions: otitis2007@hei.org ; Abstract site is open for submission until March 1, 2007. Click HERE to Register, and Click Here To Submit Abstract.

2   16th Annual general meeting and scientific conference of otorhinolaryngological society of Nigeria (ORLSON), November 21 - 24, 2007. See Here For details.

3. . Further Conference Announcement? Click Reed our Feed or Check the Conference Announcement Page

 

 
 

Text Box: Age, Systemic Dx, Vertigo & Poor prognosis in HZO...?

This brilliant paper from China (Ynag Chen et al, Hearing Research, Volume 228, Issues 1-2, June 2007, Pages 3-10) transplanted the embryonal stem cells harvested from the olfactory bulb into the cohclear nucleus, followed by intense auditory stimulation and observed that the transplanted cells migrated bi-directionally to the root of the auditory nerve, with futureimplication in retrocochlea hearing restoration if synaptic contacts between the implanted cells and auditory neurons could be established


Abstract:

The regeneration of the auditory neural system remains a challenge in hearing restoration. Acoustic signals may induce a site-specific cell replacement in the auditory system. This hypothesis was tested with grafted implantation of neural precursor cells (NPCs) along the cochlear nucleus in the adult host followed by an augmented acoustic stimulation. NPCs were obtained from the olfactory bulbs at embryonic day 14–16 and were transplanted into the inside border of cochlear nucleus. The labeled cells survived at least 2 weeks, verified by Hoechst 33342 fluorescence, and by immunostaining for a neuronal marker. In some cases NPCs had migrated directionally to the root of the auditory nerve. This observation demonstrates the survival and migration of NPCs from the olfactory bulb (OB) along the adult auditory nerve in an augmented acoustic environment following implantation.

 

     Journal Watch : The role of virtual reality in surgical training in otorhinolaryngology ,M.P. Fried, et al, 2007
 

 1.   The otolaryngologyinafrica.net resident's page has been re-designed. The current forum page is now separated from the archive page. FEEL FREE  to view the new design and recommend to your residents

 2.  From  http://www.otolaryngologyinafrica.net/grandround/  is a new addition - a recent conference poster presentation. This presentation is available at http://www.otolaryngologyinafrica.net/grandround/archive.htm .

 3.   DODA has been redesigned. Take time to view the new look of this program at the DODA Images Page... You may also download DODA Video, mobile devices edition, which can be installed on your cell phone (Note:  a multimedia cell phone is required).

 4.   otolaryngologyinafrica.net website is being re-organized to enable multi-language access. Take time to review the new HomePage. When Completed, it might be possible to assess the contents of the site in the six major languages widely spoken all over Africa

 5.  Otolaryngologyinafrica.net is experimenting with use of SMS notification of newsletter release. Feel free to air your view on this issue by emailing orl-mailer@otolaryngologyinafrica.net .

 Till Next Week, Welcome to 2007!

 Biodun

 
 

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